Volume 4, Issue 1, February 2016, Page: 1-8
Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria
Mandour H. Abdelhai, State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, Jiangsu, China
Hinawi A. M. Hassanin, State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Wuxi, Jiangsu, China
Xiulan Sun, State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, Jiangsu, China
Received: Jan. 4, 2016;       Accepted: Jan. 13, 2016;       Published: Jan. 27, 2016
DOI: 10.11648/j.bio.20160401.11      View  6104      Downloads  379
Detection of pathogenic bacteria in food is most important for food safety and quality control, and the critical step it chooses the rapid, sensitive and more economical method to extract DNA to produce high quality and decrease the time-consuming of measuring. Extraction of nucleic acids is the first step in most molecular biology studies and in all recombinant DNA techniques, but the difficult access steps and critical of analysis. Here we report, describe and compare the simple and fast methods of extraction (physical, boiling, phenol/ethanol and commercial kit) methods, from pure culture and then from beef samples. The quantity and quality of extraction methods were confirmed by polymerase chain reaction, agarose gel electrophoresis, and spectrophotometer nanodrop. Results revealed that the efficiently for all three methods were significant compared with the commercial kit, however, in pure culture the boiling method sex tract its more efficient, convenient and cheaper method for template preparation and significant when it compare with other methods while in beef samples experimental results showed that the phenol/ethanol method extract its more significantly.
DNA Extraction, Food Safety, Polymerase Chain Reaction, Pathogenic Bacteria
To cite this article
Mandour H. Abdelhai, Hinawi A. M. Hassanin, Xiulan Sun, Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria, American Journal of Bioscience and Bioengineering. Vol. 4, No. 1, 2016, pp. 1-8. doi: 10.11648/j.bio.20160401.11
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Santos Mendonça, R. C., A. M. F. Morelli, et al. (2012). "Prediction of Escherichia coli O157: H7 adhesion and potential to form biofilm under experimental conditions." Food Control 23(2): 389-396.
Kraushaar, B. and A. Fetsch (2014). "First description of PVL-positive methicillin-resistant Staphylococcus aureus (MRSA) in wild boar meat." International journal of food microbiology 186: 68-73.
Chen, X., M. Gan, et al. (2014). "Development of a rapid and sensitive quantum dot-based immunochromatographic strip by double labeling PCR products for detection of Staphylococcus aureusin food." Food Control.
Bruscolini, F., F. Barbieri, et al. (2014). "A multi-approach study of influence of growth temperature and nutrient deprivation in a strain of Aeromonas hydrophila." International journal of food microbiology 188: 1-10.
Taskila, S., M. Tuomola, et al. (2012). "Enrichment cultivation in detection of food-borne Salmonella." Food Control 26(2): 369-377.
Jenkins, M., D. Endale, et al. (2008). "Most probable number methodology for quantifying dilute concentrations and fluxes of Salmonella in surface waters." Journal of applied microbiology 104(6): 1562-1568.
Control, C. f. D. and Prevention (2011). "National diabetes fact sheet: national estimates and general information on diabetes and prediabetes in the United States, 2011." Atlanta, GA: US Department of Health and Human Services, Centers for Disease Control and Prevention 201.
Hsu, B.-M., K.-H. Huang, et al. (2011). "Evaluation of different analysis and identification methods for Salmonella detection in surface drinking water sources." Science of the Total Environment 409(20): 4435-4441.
Budiati, T., G. Rusul, et al. (2013). "Prevalence, antibiotic resistance and plasmid profiling of Salmonella in catfish (Clarias gariepinus) and tilapia (Tilapia mossambica) obtained from wet markets and ponds in Malaysia." Aquaculture 372: 127-132.
Clausen-Schaumann, H., M. Rief, et al. (2000). "Mechanical stability of single DNA molecules." Biophysical Journal 78(4): 1997-2007.
Zhang, J., J. Stewart, et al. (2000). "Economical and rapid method for extracting cotton genomic DNA." J. Cotton Sci 4: 193-201.
Somma, M. and M. Querci. (2004). "The Analysis of Food Samples for the Presence of Genetically Modified Organisms." Training course. World health organization regional office for Europe.
Di Pietro, F., F. Ortenzi, et al. (2011). "Genomic DNA extraction from whole blood stored from 15-to 30-years at− 20°C by rapid phenol–chloroform protocol: A useful tool for genetic epidemiology studies." Molecular and cellular probes 25(1): 44-48.
McOrist, A. L., M. Jackson, et al. (2002). "A comparison of five methods for extraction of bacterial DNA from human faecal samples." Journal of microbiological methods 50(2): 131-139.
Anderson, K. and S. Lebepe‐Mazur (2003). "Comparison of rapid methods for the extraction of bacterial DNA from colonic and caecal lumen contents of the pig." Journal of applied microbiology 94(6): 988-993.
Giacomazzi, S., F. Leroi, et al. (2005). "Comparison of three methods of DNA extraction from cold‐smoked salmon and impact of physical treatments." Journal of applied microbiology 98(5): 1230-1238.
Svastova, P., I. Pavlik, et al. (2002). "Rapid differentiation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis by amplification of insertion element IS901." VETERINARNI MEDICINA-PRAHA- 47(5): 117-121.
Diana, J., C. Pui, et al. (2012). "Enumeration of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in fruit juices." Int. Food Res. J 19: 51-56.
Pui, C. F., W. C. Wong, et al. (2011). "Simultaneous detection of Salmonella spp., Salmonella Typhi andSalmonella Typhimurium in sliced fruits using multiplex PCR." Food Control 22(2): 337-342.
Vetrone, S. A., M. C. Huarng, et al. (2012). "Detection of non-PCR amplified S. enteritidis genomic DNA from food matrices using a gold-nanoparticle DNA biosensor: a proof-of-concept study." Sensors 12(8): 10487-10499.
Bai, Y., M. Song, et al. (2013). "A rapid method for the detection of foodborne pathogens by extraction of a trace amount of DNA from raw milk based on amino-modified silica-coated magnetic nanoparticles and polymerase chain reaction." Analytica chimica acta 787: 93-101.
Jiang, Y., F. Gao, et al. (2010). "Changes in the bacterial communities of vacuum-packaged pork during chilled storage analyzed by PCR–DGGE." Meat science 86(4): 889-895.
Abdalhai, H. M, et al (2014) "Rapid and Sensitive Detection of Foodborne Pathogenic Bacteria (Staphylococcus aureus) Using an Electrochemical DNA Genomic Biosensor and Its Application in Fresh Beef"J. Agric. Food Chem. 62, 12659−12667.
Abdalhai, H. M, et al (2015) "Electrochemical Genosensor to Detect Pathogenic Bacteria (E.Goli O157:H7) as Applied in Real Food Samples (fresh beef) to Improve Food Safety and Quality Control"J. Agric. Food Chem. 63, 5017−5025.
Guan, Z. P.; Jiang, Y.; Gao, F.; Zhang, L.; Zhou, G. H.; Guan, Z. J., (2013). Rapid and simultaneous analysis of five foodborne pathogenic bacteria using multiplex PCR. European Food Research and Technology, 237, 627-637.
Gao, C.-y., C.-r. Tian, et al. (2014). "Phenolic composition, DNA damage protective activity and hepatoprotective effect of free phenolic extract from Sphallerocarpus gracilis seeds." International immunopharmacology 20(1): 238-247.
Nicklas, J. A. and E. Buel (2003). "Quantification of DNA in forensic samples." Analytical and bioanalytical chemistry 376(8): 1160-1167.
Browse journals by subject